Proteomic analysis of human osteoprogenitor response to disordered nanotopography.

Nanomaterials Can Influence Living Biological Systems with Nanometer Sensitivity

We show the different response of human neuroblastoma cells line (SH-SY5Y) to gold surfaces with different levels of nanoroughness, finding out that neurons are capable to sense and actively respond to these nanotopography features. Particularly, focal adhesion complexes cannot assemble onto nanostructured surfaces, leading to a marked decrease in cell adhesion. We demonstrated that nanoscale f...

Control of growth and inflammatory response of macrophages and foam cells with nanotopography

Macrophages play an important role in modulating the immune function of the human body, while foam cells differentiated from macrophages with subsequent fatty streak formation play a key role in atherosclerosis. We hypothesized that nanotopography modulates the behavior and function of macrophages and foam cells without bioactive agent. In the present study, nanodot arrays ranging from 10- to 2...

O-7: Insulin Exerts a Prosurvival Effect on Human Spermatozoa via Mechanisms That Involve the Stimulation of Akt Phosphorylation

Background: The purpose of this study was to examine the impact of Insulin (INS) on human sperm function, in light of a recent proteomic analysis indicating that these cells express the INS receptor (INSR). Materials and Methods: We used Western blot and Immunocytochemical analyses. Results: Immunocytochemical analyses confirmed the presence of INSR in human spermatozoa and localized this recep...

I-9: Proteomic Analysis of Mouse Oocytes Reveals 28 Candidate Factors of the

The contribution of inflammasome components on macrophage response to surface nanotopography and chemistry

Implantable devices have become an established part of medical practice. However, often a negative inflammatory host response can impede the integration and functionality of the device. In this paper, we interrogate the role of surface nanotopography and chemistry on the potential molecular role of the inflammasome in controlling macrophage responses. To achieve this goal we engineered model su...